Figure 5.

Northern blot analysis of TfR2 and TfR expression in mice with dietary iron deficiency or overload. Liver RNA was isolated from mice maintained on a standard (0.02% iron) diet (lanes 1 and 2), mice with dietary iron deficiency (lanes 3 and 4), and mice maintained on a diet supplemented with 2% carbonyl iron for 6 wk (lanes 5 and 6). Fifteen micrograms of total cellular RNA was electrophoresed in duplicate and blotted. Blots were hybridized with ³²P-labeled probes for mouse TfR2 (A) or mouse TfR (B). Blots were washed under high stringency, exposed to film for 18 hr with an intensifying screen, and rehybridized with a probe for β-actin (Bottom).