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Actin and vinculin double-labelled immunofluorescence images of hFOB cells cultured for 3 and 24 h on nanoislands and flat substrata taken at a 40× objective magnification. Substrata notation and scale bars are indicated. Distinct actin stress fibres and vinculin plaques as well as their colocalization were seen in cells cultured on 11 nm high nanoisland surfaces ((c), (d)), while more diffused actin and vinculin immunoreactivities on larger nanoisland substrata ((k), (l)).
