Figure 5.

(a) Electron micrograph of the surface of bone, showing a 15–20 μm microcrack and subsequent corresponding Raman images 50×50 μm (1 pixel=1 μm) of protein (1400–1440 cm⁻¹, red square), ${\text{CO}}_3^{2-}$ (1070 cm⁻¹, green square) and ${\text{PO}}_4^{3-}$ (960 cm⁻¹, blue square); (b) XRMA maps for C, O, P and Ca, the area covered by the white square corresponds to the electron micrograph in (a). Scale bar in (a) represents 50 μm and in (b) 25 μm.