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. 2006 Jul 28;91(8):2936–2942. doi: 10.1529/biophysj.106.088716

FIGURE 4.

FIGURE 4

Selected two-photon excitation fluorescence intensity images of Laurdan-labeled GUVs of the system POPC/DLPC (50:50) at T = 20°C taken during the decompression cycle from 2 kbar to 1 bar. The white arrows at 1100 bar indicate conditions where budding of small vesicles and pinching-off of lipid material occur. As the pressure is released, the GUVs start wobbling. Another interesting observation is that the GP is not homogeneous throughout the vesicle anymore.