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. 2006 Jul 28;91(8):2882–2891. doi: 10.1529/biophysj.106.087338

FIGURE 2.

FIGURE 2

DTDP-induced increase of [Zn2+]i in intact myocytes. Myocytes were loaded with fluo-4 (A–E) or mag-fura-5 (F). (A) The myocytes were exposed to 100 μM DTDP followed by washout in the absence or presence of 1 mM DTT, respectively. (B) The antagonizing effect of 10 μM TPEN on the signal induced by 1 μM ZnPyr, a specific Zn2+ ionophore. (C) The myocytes were exposed to 100 μM DTDP followed by washout in the absence or presence of 10 μM TPEN, respectively. (D) The influence of pretreatment with 10 μM TPEN on the effect of DTDP. (E) Summarized results of the effects of 100 μM DTDP and 10 μM TPEN on the fluo-4 fluorescence; n is the number of the examined myocytes. Versus control, **p < 0.01; versus DTDP alone, ##p < 0.01. (F) The effect of DTDP on mag-fura-5 fluorescence.