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Variation of the deuterium NMR quadrupole splittings of POPC membranes with the verapamil/lipid molar ratio. (A) Phosphocholine headgroup segments: (▪) α-CD2 POPC (−POCD2CH2N); (•) β-CD2-POPC (−POCH2CD2N+). (B) POPC deuterated at the cis-double bond of the oleic acyl chain: (▪) C-9′ deuteron, (•) C-10′ deuteron. Measurements at 22°C in buffer (MES 25 mM + 0.1 M NaCl, pH 5.5).
