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Titration of a 100 μM verapamil solution in 50 mM HEPES, pH 7.4 with 30 nm unilamellar lipid vesicles in the same buffer. Lipid composition is POPC/POPG (75:25 mol/mol). The injection of the lipid vesicles was in 5 μL steps. Measuring temperature 37°C. (A) Heat flow and (B) cumulative heat of reaction as a function of injection number.
