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Binding of verapamil to POPC SUVs and mixed POPC/DOTAP (94.2:15.8 mol/mol) SUVs at various salt concentrations. (○) Pure POPC SUVs; (□) POPC/DOTAP SUVs. All measurements in 50 mM Tris or HEPES buffer + various concentrations of NaCl at 37°C: The solid lines are theoretical binding isotherms calculated with the partition coefficients listed in Table 1.
