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. 2006 Sep 26;103(40):14738–14743. doi: 10.1073/pnas.0604005103

Fig. 2.

Fig. 2.

SF absorption kinetic traces from reactions of P4H·Fe(II)·αKG [±(Pro-Ala-Pro-Lys)3] containing either unlabeled (Pro-Ala-Pro-Lys)3 or deuteriated substrate (d7-Pro-Ala-d7-Pro-Lys)3 with O2. Final reaction conditions were 0.75 mM P4H, 0.50 mM Fe(II), 5 mM αKG, 5 mM ascorbate, 5 mM substrate, and 0.2 mM O2. (Upper) Absorbance at 520 nm for the reactions lacking any peptide substrate (squares) and with (Pro-Ala-Pro-Lys)3 (open circles). The solid line is a fit of the equation given in Experimental Methods to the data. (Lower) Absorbance at 320 nm in the reactions of (Pro-Ala-Pro-Lys)3 (open circles) and (d7-Pro-Ala-d7-Pro-Lys)3 (closed circles). The solid lines are fits, as described in Experimental Methods.