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Effects of aptamers on growth in selective media
| Additions to medium | pBMT119 | pBMT101 |
|---|---|---|
| None | + | + |
| ara | + | − |
| ara, thymine | + | + |
| Thymine, TMP | − | − |
| ara, thymine, TMP | − | + |
E. coli SM10 bearing the indicated plasmids were streaked onto minimal medium and incubated at 37° for 2–3 days. Plasmid pBMT119 encodes a control aptamer, and pBMT101 encodes the inhibitor of ThyA. +, growth; −, no growth; ara, arabinose; TMP, trimethoprim.
