Table 2.
Effects of various aptamers on the ThyA phenotype
| Plasmid | Additions to growth medium
|
Interpretation | ||
|---|---|---|---|---|
| Ara | Ara+Thy | Ara+Thy+TMP | ||
| pBMT | + | + | − | No effect |
| pBMT101 | − | + | + | ThyA inhibitor |
| pBMT101Q | − | + | + | ThyA inhibitor |
| pBMT101A | − | + | + | ThyA inhibitor |
| pBMT101S | − | + | + | ThyA inhibitor |
| pBMT101Oc2 | + | + | − | No effect |
E. coli SM10 bearing the indicated plasmids were streaked onto minimal medium and incubated at 37° for 2–3 days. +, growth; −, no growth; ara, arabinose; Thy, thymine; TMP, trimethoprim. Cells bearing any of these plasmids grew in minimal medium without additions.