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Two-hybrid analysis of aptamer–ThyA interaction
| α Fusion partner | λcI fusion partner | β-Galactosidase |
|---|---|---|
| ThyA | TrxA101Q | 299 (±10) |
| None | TrxA101Q | 46 (±0.6) |
| ThyA | TrxA | 100 (±1) |
| None | TrxA | 118 (±0.2) |
| Gal11P | Gal4 | 739 (±5) |
Cells expressing RNA polymerase α-subunit and λ cI fused to the indicated proteins were induced with 20 μM isopropyl β-d-thiogalactoside, and β-galactosidase activity was determined. Data shown are the mean and range of duplicates.
