Skip to main content
. 2006 Sep 26;103(40):14808–14812. doi: 10.1073/pnas.0604227103

Fig. 4.

Fig. 4.

Down-regulation of coilin does not affect cell-cycle and histone transcription. HeLa cells were transfected with either a pSUPER-FLASH-1 (shFL) or pSUPER-coilin (shCoil) or pSUPER-scrambled sequence (Cont) together with pGFP-spectrin. An aliquot of each sample was used for cell-cycle detection, mRNA, and protein extraction. (a) Western blot for the indicated proteins was performed on HeLa cells treated as described earlier for 72 h. Whereas FLASH down-regulation results in the decrease of histones H4 and H3 and NPAT levels, coilin down-regulation produces only a very modest effect on histone H4 protein levels. (b) RT-PCR for histones H4/k and H3/c using mRNA of HeLa cells treated as described earlier for 48 and 72 h. After 72 h, down-regulation of coilin produces a modest effect on histone genes mRNA levels. (c) Cell-cycle analysis of cells transfected as described earlier for 72 h. Coilin down-regulation does not affect cell-cycle regulation.