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MS demonstrating hydroxylation of endogenous IκBα. MS/MS spectra are depicted showing the N-terminally modified *CGADVNR peptide, derived by 6-exo-trig cyclization from the peptide S-alkylated with iodoacetamide. (Left) Fragments of the unhydroxylated IκBα peptide, [M+H]+ = m/z 774.3. (Right) Fragments of the hydroxylated IκBα peptide, [M+H]+ = m/z 790.3; a +16-Da shift is observed in the y-ion series, appearing at the y2 ion, which corresponds to fragments containing Asn-244.
