Figure 4.

Stimulation of endothelial cell motility by shed vesicles. (A) Vesicles shed from 1A9-VAS-3 (circles) and 1A9-VS-1 (triangles) were isolated and tested for their ability to stimulate HUVEC motility (see Figure 2). (B) Vesicles isolated from 1A9-VS-1 cells were resuspended in either PBS (open triangles) or water (filled triangles), and, after correction of molarity, tested for motogenic activity. Data (mean and SD of triplicates) represent the number of cells that migrated in 10 high-power fields.