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Q-FISH images from two sequential biopsies of a patient with a pilocytic astrocytoma. FISH with centromeric (FITC; green) and telomeric (Cy3; red) PNA probes on paraffin-embedded recurrent PLGG. DAPI was used as a counterstain with x 100 (left) and x 10 (middle) objectives. Identical images in black and white (right) with nuclei outlined in gray and telomere signals represented by dark spots. Red dots represent telomeres, and green dots represent centromeres.
