Figure 8.

GrB/scFvMEL inhibits A375DR cell invasion of Matrigel. A375DR cell aggregates were prepared as described under Materials and Methods. Cell aggregates were transferred over the Matrigel cushion and then overlaid with an additional 100 µl of Matrigel. The aggregates into Matrigel were covered with 400 µl of culture medium in the absence or presence of GrB/scFvMEL (50 nM). The aggregates were then observed daily under a light microscope (A). A375DR cells actively leave the aggregate and invade Matrigel preparation at 4 and 6 days. The treatment of A375DR cells with GrB/scFvMEL inhibits the A375DR invasion of Matrigel at 4 and 6 days. The densities of cells invading into Matrigel surrounding the aggregates were analyzed by AlphaEaseFC software, and percent invasion was calculated based on the cell densities of the two groups and standardized by the value of the nontreatment control group as 100% invasion (B).