Table 1.

Cytotoxicitiy of GrB/scFvMEL Versus MEL sFv/rGel against Various Human Melanoma Cell Lines^*.

Cell Line	gp240 Antigen (% Positive)†	IC50 (nM)
		GrB/scFvMEL	scFvMEL/rGel
A375-M	98.9	32.1 ± 7.95	22.03 ± 3.08
MEL-526	97.8	50.0 ± 4.32	40.0 ± 5.55
TXM-18L	40.9	158.3 ± 12.45	144.3 ± 9.56
TXM-1‡	4.6	>1000	>1000

^*Samples (GrB/scFvMEL and MEL sFv/rGel) were assayed using a standard 72-hour cell proliferation assay and using crystal violet staining. IC₅₀ values were calculated and demonstrated as mean ± SEM (nM).

^†Percent positive FACS analysis.

^‡No cytotoxic effect was observed at doses of up to 1000 nM.