Table 2.

Relative Proportion of Heparan [³⁵S]Sulfate Disaccharides Yielded by Degradation with Heperitinases I and II.

HS	Supernatants				Cell Extracts
	ma	Tm1	Tm5	S11	ma	Tm1	Tm5	S11
ΔUA-GlcNAc,6S	11	10	10	11	9	9	8	10
ΔUA-GlcNS	41	37	34	36	33	33	25	31
ΔUA-GlcNS,6S	29	43	41	35	36	44	44	35
ΔUA,2S-GlcNS,6S	19	11	15	18	22	14	23	24

The radioactivity of the disaccharides was measured after the separation (by paper chromatography) of compounds incubated with both heparitinases I and II. The types of uronic acid were not taken into account.

ma, melan-a; Tm1, Tm5, and S11 ,tumorigenic melanoma cell lines; ΔUA-GlcNS, O-(4-deoxy-hex-4-enopyranosyluronic acid)-(1-4)-2-sulfamino-D-glucose; ΔUA-GlcNAc,6S, O-(4-deoxy-hex-4-enopyranosyluronic acid)-(1-4)-2-acetamido-D-glucose 6-sulfate; ΔUA-GlcNS,6S, O-(4-deoxy-hex-4 enopyranosyluronic acid)-(1-4)-2-sulfamino-D-glucose 6-sulfate; ΔUA,2S-GlcNS,6S, O-(4-deoxyhex-4-enopyranosyluronic acid 2-sulfate)-(1-4)-2-sulfamino-D-glucose.