Figure 1.
Schematic of an invasion chamber. (1) A total of 120 µl of the ECM gel was added and incubated at 37°C for 30 minutes for polymerization. (2) A total of 1.5 x 105 MDA-MB-231 cells in 100 µ1 of EGM-2 were seeded on the surface of the ECM gel and incubated for 4 hours to allow cells to attach; 100 µ1 of EGM-2 alone was added to the control chambers. (3) A total of 200 µl of the ECM gel (diluted 1:2 in DMEM) was added and polymerized within 30 minutes at 37°C. (4) A total of 1.5 x 105 labeled HUVECs in 300 µl of EGM-2 were seeded and incubated at 37°C for 24 hours to allow for the attachment of HUVECs and the formation of lumen-like structures.