Table 2.
Yeast up-regulated genes identified by in silico ESTs subtraction and cDNA microarray.
| PbAEST | Acession Numbers (GenBank) | Annotated function | Number of reads | P-valuea | Fold change | Accession Number/Best-hit organism/E-value | Functional categories | |
| M | Y | |||||||
| 1422 | CA581980 | Alpha-1,2-mannosyltransferase (Alg11) | 4 | 11 | 0.019803 | 2.0 | EAL88400.1/A. fumigatus/1.0E-130 | Control of cellular organization: Cell wall and membrane |
| 4988 | CN253911 | Alpha 1,3-glucan synthase* | - | 1 | - | 5.7 | AAV52833.1/P brasiliensis/4.0E-93 | |
| 2162 | CN238153 | Putative WW domain protein (probable membrane protein) | 4 | 12 | 0.013092 | 3.6 | EAL85876.1/A. fumigatus/6.0E-17 | |
| 136 | CA582283 | Involved in cytoskeletal organization and cellular growth (verprolin)* | 4 | 10 | 0.029289 | 4.0 | NP_013441.1/S. cerevisiae/2.3 | |
| 667 | CA583397 | Adenylylsulphate kinase | 3 | 8 | 0.038949 | 2.1 | EAL90409.1/A. fumigatus/1.0E-88 | Ion transport and metabolism |
| 48 | CA582091 | ATP-sulphurylase | 10 | 18 | 0.023038 | 4.8 | EAL92915.1/A. fumigatus/0.0 | |
| 2031 | CA581274 | Outer mitochondrial membrane protein porin | 1 | 14 | 0.000207 | 1.3 | XP_323644.1/N. crassa/1.0E-108 | |
| 2724 | CA581633 | P-type Cu(2+) transporting ATPase* | 0 | 1 | -b | 3.8 | NP_009854.1/S. cerevisiae/1.7E-20 | |
| 635 | CN247312 | ATP citrate lyase | 1 | 7 | 0.014984 | 1.9 | EAL88915.1/A. fumigatus/0.0 | Energy |
| 2016 | CN242578 | ATPase inhibitor; Inh1 | 2 | 14 | 0.000835 | 2.7 | NP_010100.1/S. cerevisiae/4.0E-08 | |
| 563 | CA583982 | Cytochrome c oxidase subunit VII | 11 | 43 | 0.000002 | 2.1 | AAT77147.1/P. brasiliensis/3.0E-26 | |
| 2398 | CN240705 | Disulfide isomerase | 3 | 8 | 0.038949 | 2.1 | EAL91387.1/A.fumigatus/3.0E-61 | |
| 540 | CN240558 | Cytochrome C oxidase biogenesis protein | 0 | 5 | 0.015111 | 1.8 | XP_214182.2/R. norvegicus/1.0E-06 | |
| 578 | CA582837 | Pyruvate dehydrogenase e1 component beta subunit | 2 | 7 | 0.033994 | 1.6 | EAL86696.1/A. fumigatus/2.0E-99 | |
| 407 | CA583387 | Succinyl-CoA synthetase alpha subunit | 7 | 19 | 0.004468 | 2.6 | EAL91981.1/A. fumigatus/1.0E-155 | |
| 284 | CN239025 | Ubiquinol-cytochrome C reductase complex ubiquinonE-binding protein QP-C | 0 | 4 | 0.030475 | 1.5 | EAL90680.1/A. fumigatus/7.0E-29 | |
| 378 | CA580847 | Argininosuccinate synthase | 0 | 6 | 0.007492 | 1.7 | NP_229577.1/T. maritime/4.0E-77 | Amino acid metabolism and transport |
| 1618 | CA583639 | Aromatic-L-amino-acid decarboxylase | 1 | 33 | 0.00000 | 17.2 | EAL86509.1/A. fumigatus/0.0 | |
| 125 | CA583825 | Glycine cleavage system h protein | 4 | 9 | 0.042192 | 1.4 | EAL90537.1/A. fumigatus/6.0E-36 | |
| 1674 | CA583874 | Aldolase | 5 | 14 | 0.010368 | 37.9 | AAL34519.2/P. brasiliensis/0.0 | C-compound and carbohydrate metabolism |
| 42 | CA581699 | Phosphoglycerate kinase | 1 | 10 | 0.002512 | 2.6 | EAL90363.1/A. fumigatus/0.0 | |
| 9 | CA581893 | Beta-ketoacyl synthase (Cem 1) | 1 | 5 | 0.045709 | 2.2 | EAL87667.1/A. fumigatus/1.0E-88 | Lipid, fatty-acid and isoprenoid metabolism |
| 780 | CA581145 | GPR/FUN34 family protein | 0 | 11 | 0.000225 | 14.9 | EAL87502.1/A. fumigatus/6.0E-67 | |
| 1989 | CA581550 | Acetyl-coenzyme A synthetase (AcetatE – CoA ligase) (Acyl-activating enzyme) | 1 | 9 | 0.004605 | 2.0 | EAL89682.1/A. fumigatus/0.0 | |
| 1550 | CA582818 | NADH-cytochrome b5 reductase | 0 | 6 | 0.007492 | 5.4 | EAL88164.1/A. fumigatus/1.0E-86 | |
| 300 | CA581937 | Nucleoside diphosphate kinase | 6 | 58 | 0.00000 | 1.6 | AAP85295.1/A. fumigatus/2.0E-67 | Nucleotide metabolism |
| 547 | CA583473 | 6,7-dimethyl-8-ribityllumazine synthase | 0 | 6 | 0.007492 | 1.4 | AAD55372.1/A. fumigatus/9.0E-56 | Metabolism of vitamins, cofactors, and prosthetic groups |
| 924 | CN240624 | Coproporphyrinogen III oxidase | 2 | 7 | 0.033994 | 2.7 | EAL88456.1/A. fumigatus/0.0 | |
| 867 | CA580742 | NADH pyrophosphatase | 1 | 5 | 0.045709 | 5.7 | EAL85969.1/A. fumigatus/1.0E-159 | |
| 1490 | CA583063 | Pyridoxamine 5'-phosphate oxidase | 0 | 10 | 0.000453 | 3.5 | AAC28862.1/S. commune/2.0E-32 | |
| 447 | CA580589 | NADH:ubiquinone oxidoreductase B18 subunit | 1 | 10 | 0.002512 | 1.4 | EAL92195.1/A. fumigatus/9.0E-33 | |
| 488 | CA582788 | Exonuclease II | 1 | 5 | 0.045709 | 1.9 | EAL85993.1/A. fumigatus/1.0E-138 | Transcription, translation and ribosome structure |
| 165 | CN241393 | RNP domain protein | 3 | 13 | 0.003962 | 1.8 | EAL89070.1/A. fumigatus/5.0E-81 | |
| 2436 | CA580512 | Splicing factor u2af 35 kd subunit | 2 | 7 | 0.033994 | 2.5 | EAL86523.1/A. fumigatus/1.0E-103 | |
| 253 | CN240426 | Zinc finger, C3HC4 type | 0 | 5 | 0.015111 | 1.4 | NP_593329.1/S. cerevisiae/3.0E-10 | |
| 551 | CN239696 | Ribosomal protein L35** | 5 | 10 | 0.044755 | - | AAL08563.1/P. brasiliensis/1.0E-63 | |
| 979 | CA582579 | 60S ribosomal protein L7/L12 precursor | 1 | 8 | 0.008358 | 1.3 | EAL89813.1/A. fumigatus/4.0E-49 | |
| 175 | CA581863 | Complex I intermediatE-associated protein CIA30 precursor | 4 | 15 | 0.003399 | 5.6 | EAL92946.1/A. fumigatus/1.0E-114 | Protein fate and Secretion |
| 832 | CN242383 | Glutathione S transferase | 1 | 7 | 0.014984 | 2.0 | NP_588171.1/S. pombe/7.0E-42 | |
| 2387 | CA584103 | Non-classical export protein (Nce1) | 1 | 7 | 0.014984 | 55.6 | EAL87256.1/A. fumigatus/1.0E-29 | |
| 1823 | CA583903 | Profilin | 1 | 5 | 0.045709 | 1.3 | NP_014765.1/S. cerevisiae/8.0E-14 | |
| 4188 | CN245872 | Mating type protein (MAT1–2)* | 1 | 0 | - | 8.0 | EAL89707.1/A. fumigatus/2.0E-36 | Mating Type |
| 50 | CA581392 | Cu-Zn superoxide dismutasE-related* | 0 | 8 | 0.001842 | 2.1 | CAB97297.1/N. crassa/3.0E-30 | Virulence and oxidative stress |
| 2059 | CN241260 | Ribosome associated protein (Stm1) | 6 | 31 | 0.000007 | 1.7 | EAL92489.1/A. fumigatus/2.0E-32 | Others |
| 2005 | CA580764 | Signal peptide protein | 1 | 6 | 0.026442 | 2.3 | EAL93249.1/A. fumigatus/7.0E-68 | |
| 39 | CA581046 | Unknown | 0 | 6 | 0.007492 | 2.2 | - | |
| 33 | CA582496 | Unknown | 0 | 8 | 0.001842 | 3.1 | - | |
| 1442 | CA581846 | Unknown | 3 | 16 | 0.000836 | 4.5 | - | |
| 2399 | CA581839 | Unknown | 1 | 5 | 0.045709 | 2.5 | - | |
| 512 | CA583749 | Unknown | 0 | 6 | 0.007492 | 4.3 | - | |
| 639 | CA581506 | Unknown | 0 | 7 | 0.003715 | 1.7 | - | |
| 718 | CN247671 | Unknown | 0 | 6 | 0.007492 | 1.8 | - | |
| 765 | CA581478 | Unknown | 0 | 10 | 0.000453 | 3.9 | - | |
| 529 | CA580398 | Unknown | 1 | 5 | 0.045709 | 18.8 | - | |
a FDR = 4,8% and Q-value < 5%.
b Not significant by Audic-Claverie's method.
* Up-regulated genes confirmed by northern blotting.
** Not assayed by cDNA microarray but confirmed as up-regulated in yeast by northern blotting.