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Immunoblot analysis of HrpW, HrcJ, and HrcC proteins in Pst DC3000 and hrp mutants in hrp-inducing medium (A) and in planta (B). Conditions for bacterial growth and immunoblot analysis are described in Materials and Methods. For analysis of HrpW expression in hrp-inducing cultures, the levels of HrpW in cell-associated (C) and supernatant (S) fractions were examined.
