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. 2006 Oct 2;116(10):2799–2807. doi: 10.1172/JCI29021

Figure 2. Ebp proteins form an SrtC-dependent HMW ladder.

Figure 2

(A–D) Immunoblots of mutanolysin extracts of wild-type E. faecalis OG1RF; ebpA disruption (TX5421), ebpB disruption (TX5460), ebpC disruption (TX5448), and srtC deletion (TX5470) mutants; and ebpC disruption mutants complemented with ebpC (TX5476) or ebpC plus srtC (TX5479) probed with anti-Ebp antibodies. rec-, recombinant. (E) OG1RF mutanolysin extracts, either left untreated or treated with periodate, were probed with anti-Ebp sera. (F) Immunoblots of varying amounts of mutanolysin extracts (ME) of OG1RF grown in either TSBG or 40% horse serum and probed with anti-Ebp antibodies. The affinity-purified anti-Ebp Igs or anti-Ebp sera used in each Western blot are indicated. The srtC deletion mutant sample in A and the ebpB disruption mutant sample in B are from different gels.