Figure 3.
Myc activation in β cells in islets induces production of diffusible angiogenic factors in vitro. Islets were isolated from pIns-MycERTAM;RIP7-Bcl-xL mice and incubated in media containing either 4-OHT (Myc ON) or EtOH carrier (Myc OFF). Medium from these cultures was then used to treat HUVEC seeded in a fibrinogen sandwich for 72 h. (A) HUVEC tubule formation was assayed microscopically. Tubelike structures are indicated by arrows. Two independent and representative studies are shown. (B) The proportion of HUVEC cells incorporated into tubelike structures was determined after application of media conditioned by Myc ON or Myc OFF islets, as described in Materials and Methods.