Skip to main content
. 2006 Sep 15;20(18):2593–2604. doi: 10.1101/gad.1447106

Figure 2.

Figure 2.

Dmc1 foci in spo11Δ tid1Δ do not result from elevated Dmc1 levels, SPO11-independent chromosome breaks, or SPO11-independent ssDNA tracts. (A) Western blot analysis of Dmc1 protein levels from wild-type, tid1Δ, spo11Δ, and spo11Δ tid1Δ compared with Arp7 loading control. (B) Analysis of the integrity of meiotic chromosomes during a meiotic time course from wild-type, tid1Δ, spo11Δ, and spo11Δ tid1Δ strains by CHEF gel electrophoresis (per strain from left to right: 0, 3.5, 4, 6, and 8 h in meiosis). Note that in wild type, formation of DSBs at 3.5 h is evident, followed by restoration of chromatin integrity at 8 h. spo11Δ and spo11Δ tid1Δ mutants do not show loss of chromosome integrity. (C) Three representative spread nuclei prepared from cells collected 4 h after transfer to sporulation medium. The relevant genotype is indicated. Dmc1 foci are shown in green, and RPA foci are shown in red.