Figure 5.

The FASPS mutation leads to a premature nuclear clearance. (A) PER2 variant-expressing NIH3T3 cell lines were treated with the protein translation inhibitor CHX. Cells were fixed at the indicated time points after treatment and immunostained with anti-V5 and fluorescently labeled secondary antibody. Shown are representative fields of two experiments. Bar, 20 μm. (B) HEK293 cells were transiently transfected with either PER2 wild type, mut-7, or a mut-7 variant, whose nuclear localization signals 1 and 3 (mut-7 NES13) were mutated. CKIε was coexpressed where indicated, and PER2 protein abundance was detected by Western blotting.