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. 2006 Oct 1;20(19):2673–2686. doi: 10.1101/gad.1451806

Figure 1.

Figure 1.

BM remodeling and transmigration by cancer cells. (A, left) Mesothelial BM (rat peritoneum) shown by TEM (red arrows demarcate BM). (Right) SEM (BM) highlights the sheet-like structure of the BM overlaying the stromal matrix (indicated by an asterisk). Immunostaining for type IV collagen and laminin (horizontal arrows) reveals the two BM layers (i.e., the upper and lower surfaces of the peritoneum are lined by BMs). Insets show the epithelial BM deposited by MDCK cells as visualized by TEM and SEM. Bars for TEM, SEM, and light micrographs are 1, 20, and 100 μm, respectively. (B) MDA-MB-231 cells cultured atop the peritoneal BM at 0 and 8 d as assessed by TEM. BM (highlighted by red arrows) is breached by carcinoma cells after an 8-d culture period. Inset shows an H&E section of carcinoma cells at 0 d. Bars for TEM and light micrographs are 1 and 200 μm, respectively. (C) SEM of peritoneal BM stripped of overlying MDA-MB-231 cancer cells immediately after plating (0 d), or after an 8-d culture period (8 d). Bar, 10 μm. (D) Representative H&E cross-sections of the peritoneum after an 8-d culture period. The number of invasive cells per field are quantified in H&E-stained cross-sections with error bars indicating the standard error of mean of five or more experiments.