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. 2006 Oct 1;20(19):2687–2700. doi: 10.1101/gad.1447006

Figure 4.

Figure 4.

p53 associates with the Lats2 promoter in vivo. (A) Location and sequence of predicted p53REs in the Lats2 gene. Numbers represent nucleotide positions relative to the accepted 5′ end of the Lats2 mRNA transcription start site (TSS). Open boxes represent untranslated regions, and dark boxes represent coding regions. (B) ChIP analysis of DNA derived from stably transfected U2OS/sip53 or U2OS/siControl (siLacZ) cells. Cells were treated with 0.2 μg/mL nocodazole (noc) for 3 or 6 h or with 1 μM doxorubicin (Doxo) for 6 h. p53-bound chromatin was precipitated using CM1 polyclonal antibody (Novocastra). Chromatin-derived DNA was subjected to real-time PCR analysis with primer pairs bracketing the 5′ p53RE of the p21 gene and the Lats2 p53RE-I (see A). Values represent the ratio of the value obtained for bound DNA over that obtained for total input (chromatin before immunoprecipitation) DNA for each PCR primer pair (bound/input).