Figure 1.
(a) Southern blot analysis of genomic tail DNA from p35 transgenic (p35) vs. wild-type (WT) mice. DNA was digested with XbaI and hybridized to a 32P-labeled full-length p35 DNA probe. Lanes 1, 2, 3, 5, and 7, p35 transgenics; lanes 4 and 6, nontransgenics. (b) Northern blot of whole brain RNA from three lines of transgenics (3.3, 3.6, and 8.4) vs. wild-type mice; blots were hybridized with 35S-labeled full-length p35 riboprobe. Lanes 2–4, p35 transgenic lines; lane 1, wild-type. (Expected p35 transcript size = 2.0 kb). (c) Analysis of mRNA distribution in brains of transgenic mice by in situ hybridization of sagittal sections with a 35S-labeled riboprobe specific for p35. (d) Immunocytochemistry with polyclonal p35 antibody on sagittal sections of brains from p35 transgenics. HC, hippocampus; SN, substantia nigra; and CB, cerebellum.