(a) PI-stained cultures 24 h after treatment with KA. Cultures from wild type (1 and 3) and p35 transgenics (2 and 4) after treatment with either 10 or 25 μM KA, respectively. (Bar = 500 μm.) (b) Cell viability of p35 transgenic vs. wild-type control cultures after treatment with KA as measured by LDH release. Values represent the means ± SD from three experiments. (*, P < 0.01.) (1) LDH efflux 24 h after KA treatment; values are reported as % wild type treated with 40 μM KA. WT, wild type; HT, p35 heterozygote; and HM, p35 homozygote. Shaded bars, 40 μM KA; open bars, 20 μM KA; filled bars, 10 μM KA. (2) Time course of LDH release after treatment with 25 μM KA; LDH is reported as units/min. Filled bars, KA-treated wild type; horizontal striped bars, KA-treated p35 transgenic; open bars, untreated wild type; and vertical striped bars, untreated transgenic.