Abstract
Extracts of erythrocytes infected with Plasmodium falciparum have been prepared from densely infected human placental tissue. Gel-filtration of such extracts on Sephadex G-200 readily separated two types of antigen, designated α and β, respectively. In addition, soluble antigens were detected in the sera of several children and parturient mothers immediately following attacks of malaria. These soluble antigens were found to be immunochemically similar to the α group of placental antigens. Combined histochemical and immunochemical tests indicated that the α antigens were probably proteins.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chavin S. I. Studies on the antigenic constituents of Plasmodium berghei. I. Immunologic analysis of the parasite constituents. II. Fractionation of the parasite constituents. Mil Med. 1966 Sep;131(9 Suppl):1124–1136. [PubMed] [Google Scholar]
- Cox H. W. A factor associated with anemia and immunity in Plasmodium knowlesi infections. Mil Med. 1966 Sep;131(9 Suppl):1195–1200. [PubMed] [Google Scholar]
- DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
- Diggs C. L. Immunodiffusion studies of Plasmodium berghei: interactions of an extract of the erythrocytic forms with rabbit antisera. Exp Parasitol. 1966 Dec;19(3):237–248. doi: 10.1016/0014-4894(66)90073-7. [DOI] [PubMed] [Google Scholar]
- FAHEY J. L., MCLAUGHLIN C. PREPARATION OF ANTISERA SPECIFIC FOR 6.6 S GAMMA-GLOBULINS, BETA 2A-GLOBULINS, GAMMA-1.-MACROGLOBULINS, AND FOR TYPE I AND II COMMON GAMMA-GLOBULIN DETERMINANTS. J Immunol. 1963 Oct;91:484–497. [PubMed] [Google Scholar]
- GRABAR P., WILLIAMS C. A. Méthode permettant l'étude conjuguée des proprietés électrophorétiques et immunochimiques d'un mélange de protéines; application au sérum sanguin. Biochim Biophys Acta. 1953 Jan;10(1):193–194. doi: 10.1016/0006-3002(53)90233-9. [DOI] [PubMed] [Google Scholar]
- McGregor I. A., Hall P. J., Williams K., Hardy C. L. Demonstration of circulating antibodies to Plasmodium falciparum by gel-diffusion techniques. Nature. 1966 Jun 25;210(5043):1384–1386. doi: 10.1038/2101384a0. [DOI] [PubMed] [Google Scholar]
- McGregor I. A., Turner M. W., Williams K., Hall P. Soluble antigens in the blood of African patients with severe plasmodium falciparum malaria. Lancet. 1968 Apr 27;1(7548):881–884. doi: 10.1016/s0140-6736(68)90237-7. [DOI] [PubMed] [Google Scholar]
- OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
- Turner M. W., McGregor I. A. Studies on the immunology of human malaria. II. Characterization of antibodies to Plasmodium falciparum infections. Clin Exp Immunol. 1969 Jul;5(1):17–27. [PMC free article] [PubMed] [Google Scholar]
- URIEL J., AVRAMEAS E. [Characterization of the nucleoproteins and nucleic acids after electrophoresis and immunoelectrophoresis on starch]. C R Hebd Seances Acad Sci. 1961 Mar 6;252:1524–1526. [PubMed] [Google Scholar]
- Weir D. M. The immunologicial consequences of cell death. Lancet. 1967 Nov 18;2(7525):1071–1073. doi: 10.1016/s0140-6736(67)90342-x. [DOI] [PubMed] [Google Scholar]
- ZUCKERMAN A. THE ANTIGENIC ANALYSIS OF PLASMODIA. Am J Trop Med Hyg. 1964 Jan;13:SUPPL–213. doi: 10.4269/ajtmh.1964.13.209. [DOI] [PubMed] [Google Scholar]