Figure 2.

Selection from the scFv-bearing bacteriophage library. (A) The library of bacteriophage-displayed scFvs was propagated and scFv expression was induced. For each round of selection, 10¹¹-10¹² bacteriophage were placed in a selection tube whose surface had been coated with a hair-cell lysate. After incubation, extensive washing removed unbound bacteriophage. Bound bacteriophage were eluted and used to infect bacteria for propagation and expression of the enriched clones. The eluted bacteriophage subsequently were used in the next round of selection. For simplicity, we do not depict the preincubation step for removal of bacteriophage that bound to plastic or blocking reagent. (B) BstNI digestion patterns of 24 randomly selected clones indicate that a single clone, indicated by *, was enriched as a result of the antibody fragment that it expressed. Only those clones that had inserts amplifiable by the PCR are shown. Size markers are as indicated in Fig. 1.