Western blot analysis of blood mononuclear cell lysates (5 μg total protein) using affinity-purified rabbit polyclonal antibodies to GRHPR, shown in top panel. A monoclonal mouse antibody to human α-actin shows protein loading in bottom panel. Lane 1, purified recombinant human GRHPR protein; 2, PH1; 3, PH1; 4, PH2; 5, PH2; 6, BMC from normal adult subject; 7, PH2. All samples were run on the same gel and blotted to the same membrane.