Table I.

Cytokine secretion by draining lymph node cells isolated from UV-irradiated hapten-sensitized mice^a

	pg/ml/106 Cells
Treatmentb	IL-12p70	IL-10
FITC	15.2 ± 0.7	BLDc
FITC + LPS/anti-CD40	25.2 ± 4.9	72.0 ± 17.0
UV + FITC	11.2 ± 1.3	BLD
UV + FITC + LPS/anti-CD40	20.6 ± 3.0	166 ± 21.0d

^aMice were exposed to UV (15 kJ/m²; dorsal skin) and 4 days later were sensitized with hapten (0.5% FITC, abdominal skin). Eighteen hours later, FITC⁺ cells were isolated with magnetic beads.

^bThe cells were cultured in complete RPMI 1640 with or without LPS (1 μg/ml) and anti-CD40 mAb (5 μg/ml) for 24 h. Cytokines present in the supernatant were determined by ELISA.

^cBelow the limit of detection (15 pg/ml).

^dValue of p < 0.01 vs FITC + LPS/anti-CD40; two-tailed Student’s t test.