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. Author manuscript; available in PMC: 2006 Sep 28.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2006 Jan;47(1):151–157. doi: 10.1167/iovs.05-0351

Figure 4.

Figure 4

Western blot analysis. (A) LY294002, SR13668, and U0126 abolished phosphorylation of Akt at Thr308. LY294002 and SR14668 completely, but U0126 partially, abolished phosphorylation of Akt at Ser473. SR13668 completely but LY29004 and U0126 partially eliminated FKHRL1 phosphorylation at Thr32. Among all inhibitors, only U0126 abolished phosphorylation of p44/42 MAPK at both Thr202 and Tyr204.(B) Phosphorylation at Thr180/Tyr182 of p38 MAPK was expressed by cells expanded on plastic, but was markedly downregulated in those expanded on intact AM and was abolished with addition of 10 μM SB203580. (C) Phosphorylation of Thr183/Tyr185 of JNK MAPK was expressed by cells expanded on plastic, but was abolished in those expanded on intact AM, with or without addition of JNK inhibitor 1.