Table 1.

Purification of GnRH peptides from the gonads of C. intestinalis

	Volume, ml	Peptides, mg/ml	Peptides, total mg	Total peptide recovery, %
Step 1: PCA homogenization	520.0	2.41	1,253.2	100
Step 2: C-18 extraction column	200.2	0.57	115.8	9.24
Step 3: Filtration at 10,000 Dal	210.0	0.064	13.4	1.07
Step 4: Filtration at 1,000 Dal	1.0	4.98	4.98	0.39
Step 5: HPLC chromatography
cGnRH-I-like peak	0.2	6.02	1.20	0.096
mGnRH-like peak	0.2	4.91	0.98	0.078

Results obtained from 100-g gonads.