Table 2.
Amino acid composition of acid hydrolysate of peptides purified from early- and late-eluting HPLC peaks (peaks 1 and 2) of C. intestinalis gonadal extract
| Amino acid | nmol/μg | Amino acid ratio | Integral residues |
|---|---|---|---|
| Peak 1 peptide (cGnRH-I-like) | |||
| Glutamic acid | 2.01 | 2.20 | 2 |
| Hystidine | 0.97 | 1.06 | 1 |
| Tryptophan | 0.89 | 0.97 | 1 |
| Serine | 0.92 | 0.98 | 1 |
| Tyrosine | 1.01 | 1.11 | 1 |
| Glycine | 1.98 | 2.17 | 2 |
| Leucine | 0.91 | 1.00 | 1 |
| Proline | 1.01 | 1.10 | 1 |
| Ammonium ion | 1.88 | 2.06 | 2 |
| Peak 2 peptide (mGnRH-like) | |||
| Glutamic acid | 1.01 | 1.08 | 1 |
| Hystidine | 0.99 | 1.06 | 1 |
| Tryptophan | 0.93 | 1.00 | 1 |
| Serine | 0.90 | 0.96 | 1 |
| Tyrosine | 0.97 | 1.01 | 1 |
| Glycine | 1.96 | 2.10 | 2 |
| Leucine | 0.93 | 1.00 | 1 |
| Arginine | 1.02 | 1.09 | 1 |
| Proline | 1.00 | 1.07 | 1 |
| Ammonium ion | 0.97 | 1.04 | 1 |
Molar ratios of amino acids were normalized to 1.0 for leucine.