Table 1.

Effect of leptin replacement on lymphocyte subpopulations in the thymus

Thymocyte population	ob/ob	Leptin-replaced ob/ob	+/?
Total	40.50  ±  13.00**	134.50  ±  24.39‡	87.50  ±  12.81
CD4+ CD8+	22.65  ±  8.20**	84.79  ±  18.76‡	55.13  ±  9.80
CD4+ CD8−	2.99  ±  1.05**	12.23  ±  2.17‡	6.44  ±  1.86
CD4− CD8+	2.22  ±  1.13	3.73  ±  1.38	3.48  ±  1.21
CD4− CD8−	1.13  ±  0.24**	2.86  ±  0.45‡	1.64  ±  0.20

ob/ob mice received two daily injections of either saline (ob/ob) or leptin (leptin-replaced ob/ob) for 10 days. +/? mice were left untreated. After treatment, the thymus was harvested and processed for FACS analysis as indicated in Materials and Methods. Data are mean ± SEM of four mice per group. **, P < 0.01 vs. either leptin-replaced ob/ob or +/? mice; ‡, P < 0.05 vs. +/? mice by factorial ANOVA.