Table 2.
Relative solubility of 14C product formed in in vitro enzyme assays
| Treatment |
14C-insoluble material, dpm
|
||||
|---|---|---|---|---|---|
| Wild type (AX4) | dcsA− (DG1099) | dcsA− (DG1128) | dcsA− (TL127) | Rescued strain (TL128) | |
| 66% EtOH | 17,167 ± 386 | 244 ± 26 | 165 ± 26 | 177 ± 7 | 13,314 ± 525 |
| (100%) | (100%) | ||||
| 0.5 M KOH | 10,166 ± 368 | 94 ± 64 | 0 | 0 | 9,742 ± 141 |
| (59%) | (73%) | ||||
| Acetic-nitric | 13,209 ± 1,118 | 4 ± 18 | 1 ± 22 | 0 | 9,165 ± 167 |
| (77%) | (69%) | ||||
The values represent the means of triplicate reactions of a single experiment and the SD of those means. The numbers in parentheses represent the percentage of the retained activity for the given treatment compared with the retained activity after the 66% ethanol treatment.