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ITC data for complex formation. (a) Trace of the isothermal titration of HNF1β with a recognition target DNA. The experiment was performed in 20 mM imidazole pH 8.0 and 50 mM NaCl at 303 K. The concentrations of reactants are 0.12 mM HNF1β and 4 µM dsDNA. (b) Binding isotherm obtained from the experiment shown in (a). The association constant and the enthalphy and entropy of association are presented in the enclosed box.
