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Fluorescence in situ hybridization of metaphase spreads from a pool of HepG2 selected in the presence of hygromicin B. Cells were selected on coinfection with HDRA1034 and HDFB1, a helper-dependent vector carrying ITR(GFP/Hygro) cassette. Metaphase spreads were hybridized following the conditions described in ref. 4 with a GFP/hygromicin (red) and an AAVS1 probes (yellow). Colocalization of both probes on chromosome 19 is indicated by an arrow.
