Table 1.
Infectivity of Ad5 genomic plasmid carrying the rep gene
| Vector | Experiment
|
Rep activity | |
|---|---|---|---|
| 1 | 2 | ||
| pLBT7-Rep | 0 | 0 | ND |
| pLBT7-Rep78 | 4 | 6 | + |
| pLBT7-RepΔATG | 0 | 0 | ND |
| pLBT7-Rep78ΔATG | 15 | 18 | − |
| Control | 22 | 26 | − |
Data given as number of plaques obtained. Plaques were observed 10 days after 293 transfection. Plaque isolates were amplified through four serial passages on 293 cells and then evaluated for Rep activity in rescue and replication experiments as described in Materials and Methods. All AdLBT7-Rep78 isolates were positive for Rep enzymatic activity. ND, not determined.