FIGURE 1.
Effect of enzyme and terminator on chain termination. (A) Map of relevant SqKV1.1A sequence used as a template for the assay. Primer sequence is boxed. Asterisks denote editing sites (A → I) relevant in Figure 4. Arrow 1 indicates the first instance of the terminator (intended stop) and arrow 2 indicates the second instance (run through). (B) Fluorescence scan of a polyacrylamide gel containing SqKV1.1A assay products resulting from different polymerase–terminator combinations. Theseq = Thermosequenase; Ther = Therminator; Vent = Vent exo(−); ddG = dideoxy guanosine. Arrows correspond to fragments generated by stops indicated in A.