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Utility of assay on natural substrates. (A) Fluorescence scan of a polyacrylamide gel containing assay products of cDNA and gDNA templates. SqKV1.1A was amplified from squid giant fiber lobe cDNA and from gill tissue gDNA. Syr ndhB was amplified from green leaf tissue cDNA and gDNA. (B) Quantification of the natural substrate assay in A compared with sequencing individual cDNAs.
