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. 1999 Mar 16;96(6):2639–2644. doi: 10.1073/pnas.96.6.2639

Figure 4.

Figure 4

Characterization of SMRT amino terminus. (A) The schematic illustrates the various GAL4-SMRT fusions constructed, their corresponding amino acid numbers, and repressing ability. The patterns of colored and striped regions are described in the Fig. 2B legend. The GAL4-DBD is depicted as a gray oval. The fold repression represents the ability of the GAL-4-SMRT constructs to repress the activity of a GAL4-TK-luc reporter in cotransfection experiments and is relative to GAL-DBD alone. (B) Representative data comparing the repressing abilities of various regions of the m-SMRT N terminus. The constructs are shown in A. Amino acids 1–301 correspond to RD1, 427–663 to the SANT region, 736-1031 to RD2, and 1–1031 to the full m-SMRT N terminus. (C) Representative data narrowing down the RD2 region to a 150-aa core from 845 to 986. (D) Representative data demonstrating the differing repression abilities of the two m-SMRT isoforms. GAL4-SMRTβ (1–85) corresponds to RD1 with the deletion shown in Figs. 1 and 4A. GAL4-SMRTβ (1–813) corresponds to the full N terminus of m-SMRT with the β isoform deletion.