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Orp4 protein is expressed throughout the cell cycle. Fission yeast cells expressing epitope-tagged Orp4 protein were blocked in the G1, S, G2, or M phases of the cell cycle as described in Materials and Methods. Extracts prepared from blocked cells were fractionated by SDS/PAGE and Orp4 protein was detected by Western blot analysis. The same Western blots were probed for tubulin protein as a control for equal loading of the gels.
