Figure 2.

Protein dissection of the Ebola GP2 ectodomain. Shown is a portion of a reverse-phase HPLC chromatogram of a sample of Ebo-95.3 digested with protease K for 2 hr in 20 mM Tris, pH 8.7. The HPLC eluant was analyzed directly with an on-line mass spectrometer (Finnigan-MAT LCQ), and the fragments (indicated with residue numbers) were assigned by matching observed masses with a list of all possible predicted fragment masses (see text). Fragments corresponding to peptides described in this paper are labeled with parentheses.