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. 1999 Mar 16;96(6):2668–2673. doi: 10.1073/pnas.96.6.2668

Figure 3.

Figure 3

The promoter sequence influences transcriptional activation elicited by nonclassical activators. (A) Transcriptional activation elicited by LexA derivatives elicited at chromosomally integrated (at the ura3–52 locus) reporter templates bearing either the GAL1 or PHO5 promoters. Both templates bear two LexA binding sites upstream of the respective TATA element, but on template A the DNA separating these sites from the ATG is taken from the GAL1 gene, and the corresponding sequence on template B is from the PHO5 gene. The distances between the LexA sites and the TATA elements are those found naturally at the GAL1 gene (191 bp; template A) and the PHO5 gene (133 bp; template B). All the LexA constructs were expressed from the ADH1 promoter on a 2 μ vector. Transcriptional activity was measured as β-galactosidase units in yeast strains YAG22 (template A; white bars) and YAG35 (template B; black bars). (B) Primer extension analysis. The cDNA-labeled lacZ denotes transcripts from the GAL1∷lacZ reporter of YAG22.