(A) HPLC separation of hCG β-core fragment, human urinary AVL, and AVR from a crude β-core preparations. The elution profile of a representative reverse-phase liquid chromatogram of 2.5 mg run is shown. Details are described in Methods. (B) Characterization of AVL and AVR. SDS/PAGE was carried out on crude β-core (Sephadex G100 gel chromatography fractionated), fractions P1-P7 of C18 reverse-phase HPLC, pure β-core, AVL, AVR, lysozyme, and RNase. Each lane contains about 5–10 μg of sample. The presence (+) or absence (−) of anti-HIV, lysozyme, and RNase activities are shown under the SDS/PAGE pattern. Proteins smaller than 11 kDa were not separated in this SDS/PAGE system.