Table 1.
N-terminal amino acid sequence of hCGβ-core fractions
| Fractions | SDS/PAGE position | Amino acid sequence* | Analogous proteins |
|---|---|---|---|
| P1 & P2 | <11 kDa | RPR(C)RPI(N)ATLAVEK | β-core |
| VV(C)NYRDVRFESIRL | |||
| P5 & P6 | 18.5 kDa | KPPQFTWAQWFETQHI(N) | Human RNase U |
| P5 & P6 | 18 kDa | PQFTWAQWFETQHI(N) | Human RNase U |
| P5 & P6 | 14 kDa | VFER(C)ELARTLKRLG | Human lysozyme C |
| β-core (G75) | 23 kDa | KESRAKKFQRQHMDS | Human RNase A |
*
(C) and (N) were not identified during sequencing by the Edman degradation. The absence of (N) at the indicated position is consistent with glycosylation.